In our laboratory we test for antibodies by the SeraSpot, IFA and ELISA techniques. In general, the SeraSpot has a higher sensitivity and specificity in detecting Borrelia antibodies compared to the ELISA and IFA test technique.
However the development of Borrelia burgdorferi-specific antibodies can be avoided and suppressed by antibiotic therapies in recent infections. These cases can be detected by the EliSpot for tick-borne infections.
New retrospective study confirming the Lyme SeraSpot as a reliable and robust test system:
"Our evaluation data confirm that new multiplex assay generations such as SeraSpot®Anti-Borrelia
IgG/IgM are reliable and robust test systems suitable for application as confirmatory tests for serodiagnosis of
Lyme borreliosis. "
Von Baehr et al.: Evaluation of a New Multiparametric Microspot Array for Serodiagnosis of Lyme Borreliosis in: Clin. Lab. 2015;61